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MyC-CaP Mus musculus│prostate

MyC-CaP Mus musculus│prostate
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  • 产品名称：MyC-CaP Mus musculus│prostate
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简单介绍
MyC-CaP Mus musculus│prostate
产品描述
ATCCCRL-3255 MyC-CaP
基本信息
资源名称 MyC-CaP
种属 Mus musculus prostate
形态 epithelial-like
提供形式 frozen
致病类型 prostate cancer
**等级 1
应用领域 Study of prostate cancer progression from androgen dependence to a hormone-refractory or androgen-independent stage
培养方法
培养基 The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
传代方法 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC® 30-2200) or 0.05% Trypsin – 0.02% EDTA for Primary Cells (ATCC® PCS-999-003) solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: 1:5 to 1:20 is recommended.
Medium renewal: 2 to 3 times a week
生长条件 Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
生长特性 adherent
存储条件 liquid nitrogen vapor phase;complete growth medium, 90%; DMSO, 10%
详细说明
描述 The cells have an amplified androgen receptor gene despite no prior exposure to androgen withdrawal. These cells express wild-type androgen receptor (AR), retain androgen-dependent transgene expression and demonstrate androgen-dependent growth on soft agar and in mice.
Age 16 months
Gender male
Strain FVB; Hi-Myc transgenic
Derivation The MyC-CaP cell line was derived from a genetically engineered mouse prostate cancer removed from an animal that was never exposed to hormone ablation.
Receptor Expression wild-type androgen receptor (AR)
Seeding Density 5 x103 to 1 x 104 viable cells/cm2
Population Doubling Time 18 hours
Year of Origin 2005
Restrictions This product's use is governed by the MTA and the following Contributor Rights (1) Contributor will be informed of all customers that purchase this product; (2) For information on executing a license to use this product for research use (for-profit entities) or Commercial·Use (for-profit and non-profit entities); contact UCLA Office of Intellectual Property at 310-794-0558 or innovation@research.ucla.edu.
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