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上海乾思生物科技有限公司
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美国ATCC
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TC1 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myelo
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TC1 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myelo
产品资料
TC1 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myelo
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产品名称:
TC1 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myelo
产品型号:
产品展商:
乾思生物中心
产品文档:
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简单介绍
TC1 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)│
产品描述
ATCC CRL-2493 TC1
基本信息
资源名称
TC1
种属
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
类型
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
形态
lymphoblast
提供形式
冻存管
**等级
1
模式菌株
未知
说明书
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培养方法
培养基
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
传代方法
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 5 x 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
生长条件
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
生长特性
suspension, suspension
存储条件
liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
详细说明
Derivation
Animals were immunized with purified exotoxin A of Pseudomonas aeruginosa.
Spleen cells were fused with Sp2/0-Ag14 cells.
It displays specificity for SDS-denatured exotoxin A from the strain PA103 and its derivative PA103/29 but does not react with exotoxin from strain PAO1 or its derivative PAO-PR1.
Genes Expressed
immunoglobulin; monoclonal antibody; against an idiotypic determinant on TC-31 monoclonal antibody (against exotoxin A)
Cellular Products
immunoglobulin; monoclonal antibody; against an idiotypic determinant on TC-31 monoclonal antibody (against exotoxin A)
Isotype
mouse IgG1 kappa , IgG1; kappa light chain
Year of Origin
1984
Restrictions
This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.
描述
The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa.
It displays specificity for SDS-denatured exotoxin A from the strain PA103 and its derivative PA103/29 but does not react with exotoxin from strain PAO1 or its derivative PAO-PR1.
The antibody shows high specificity and reactivity for denatured exotoxin A in immunoblotting and ELISA *****s.
Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro ***** but only TC31 protected mice infected with strain PA220 in an in vivo model.;Animals were immunized with purified exotoxin A of Pseudomonas aeruginosa.
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa.
It displays specificity for SDS-denatured exotoxin A from the strain PA103 and its derivative PA103/29 but does not react with exotoxin from strain PAO1 or its derivative PAO-PR1.
The antibody shows high specificity and reactivity for denatured exotoxin A in immunoblotting and ELISA *****s.
Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro ***** but only TC31 protected mice infected with strain PA220 in an in vivo model.
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