产品 资讯
请输入产品名称
噪声分析仪 纺织检测仪器 Toc分析仪 PT-303红外测温仪 转矩测试仪 继电保护试验仪 定氮仪
首页 产品 专题 品牌 资料 展会 成功案例 网上展会
词多 效果好 就选易搜宝！
首页 >>> 产品目录 >>> 美国ATCC
仪表展览网 >>> 展馆展区 >>> CDC:V023:Clone 1 Acanthamoeba│sp. 14 > CDC:V023:Clone 1 Acanthamoeba│sp. 14

产品资料

CDC:V023:Clone 1 Acanthamoeba│sp. 14

CDC:V023:Clone 1 Acanthamoeba│sp. 14
  • 如果您对该产品感兴趣的话，可以
  • 产品名称：CDC:V023:Clone 1 Acanthamoeba│sp. 14
  • 产品型号：
  • 产品展商：乾思生物中心
  • 产品文档：无相关文档
简单介绍
CDC:V023:Clone 1 Acanthamoeba│sp. 14
产品描述
ATCC50714 CDC:V023:Clone 1
基本信息
资源名称 CDC:V023:Clone 1
种属 Acanthamoeba sp. 14
分离基物 axenic clone derived from bacterized strain CDC:V023, isolated from the eye of an ***** human female from North Carolina with Acanthamoeba keratitis, ATCC, 1992
提供形式 frozen
**等级 2
模式菌株 no
培养方法
培养基 Medium 712: PYG w/ Additives
传代方法 Protocol: ATCCNO: 30135 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
生长条件 Max Temperature: 30.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 30135 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
存储条件 1. To achieve the best results set up cultures with several different inocula (e.g. 0.25 ml, 0.5 ml, 1.0 ml). Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. 2. If the cell concentration exceeds the required level do not centrifuge, but adjust the concentration to between 2 x 106 and 2 x 107cysts/ml with fresh medium. If the concentration is too low, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield the desired concentration. 3. While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows: Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath. Allow the DMSO to solidify. Add the required volume of refrigerated medium. Dissolve the DMSO by inverting the tube several times. *NOTE: If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium. 4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/ml and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. 5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) 7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. 8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial. 9. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 712 in a T-25 tissue culture flask or plastic 16 x 125 mm screw-capped test tube. Incubate at 25°C.
详细说明
Year of Origin 1992
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
  • 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
  • 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报
产品留言
标题
内容
联系人
联系电话
电子邮件
公司名称
联系地址
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!