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pOSEX5A

pOSEX5A
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简单介绍
pOSEX5A
产品描述
ATCC87214 pOSEX5A
基本信息
资源名称 pOSEX5A
种属
提供形式 freeze-dried
**等级 1
应用领域 expression vector
vector permitting construction of fusion proteins
培养方法
培养基 ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
生长条件 Temperature: 37.0°C
详细说明
描述 Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
Absence of a stop codon between the proV' sequence and the ATG start codon allows production of a fusion protein between ProV and the cloned insert. Insertion of a stop codon at this junction may reduce the yield of recombinant protein.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX3 (ATCC 87212) by replacement of the multiple cloning site with a ribosome , intitiation codon and multiple cloning site from pTrc99A.
One of three vectors (ATCC 87214-87216) differing only in the reading frame of the multiple cloning site.
Vector Information Size (kb) 4.4429998397827150
Vector pOSEX5A (plasmid)
Promoters Promoter for expression proU
Construction pOSEX3
Marker(s)ampR
Construct size (kb) 4.442999839782715
Features initiation codon ATGmarker(s) ampRpromoter for expression proUreplicon pMB1replicon rop (copy number control)MCS NcoI...HindIIItranscription terminator rrnB T1transcription terminator rrnB T2coding sequence proV 5' sequence
Shipping Information Freeze dried E. coli containing the plasmid.
Attachments Vector Map
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