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仪表展览网 >>> 展馆展区 >>> Mv 1 Lu (NBL-7) Neovison vison, American Mink│lung > Mv 1 Lu (NBL-7) Neovison vison, American Mink│lung

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Mv 1 Lu (NBL-7) Neovison vison, American Mink│lung

Mv 1 Lu (NBL-7) Neovison vison, American Mink│lung
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  • 产品名称：Mv 1 Lu (NBL-7) Neovison vison, American Mink│lung
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简单介绍
Mv 1 Lu (NBL-7) Neovison vison, American Mink│lung
产品描述
ATCC CCL-64 Mv 1 Lu (NBL-7)
基本信息
资源名称 Mv 1 Lu (NBL-7)
种属 貂肺上皮细胞
形态 epithelial
提供形式 冻存管（ATCC）
**等级 1
模式菌株 未知
应用领域 This cell line is a suitable transfection host and is useful for focus forming *****s for murine and feline sarcoma viruses.
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培养方法
培养基 The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
传代方法 Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
生长条件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
生长特性 adherent
存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
详细说明
Age near term fetus
Gender male and female mixed
Karyotype Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.
Derivation The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink.
Clinical Data male and female mixed
Virus Susceptibility Herpes simplex virus Reovirus 3 Vaccinia virus Vesicular stomatitis New Jersey virus
Virus Resistance adenovirus 5; coxsackievirus A9, B5; poliovirus 2
Year of Origin May, 1964
Cross References Nucleotide (GenBank) M64428 Mustela vison TI1 mRNA, complete cds.
Nucleotide (GenBank) J02248 mink cell focus-forming 247 provirus clone 1 ltr.
Nucleotide (GenBank) J02250 mink cell focus-forming 247 provirus clone 2 ltr(seg 1).
Nucleotide (GenBank) J02251 mink cell focus-forming 247 provirus clone 2 ltr(seg 2).
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