白细胞激活黏附因子(ALCAM)检测试剂盒 48T/96T盒
产品简介
白细胞激活黏附因子(ALCAM)检测试剂盒用于测定血清，血浆及相关液体等样本，例如适合检测包括血清、白细胞激活黏附因子(ALCAM)检测试剂盒血浆、尿液、胸腹水、灌洗液、细胞培养上清、组织匀浆等本标本。产品种类齐全、质量可靠、价格优惠、灵敏度高、效果稳定、易保存、操作简单。
产品详细信息
白细胞激活黏附因子(ALCAM)检测试剂盒
ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | YBA002Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.059ng/mL. |
Specificity
白细胞激活黏附因子(ALCAM)检测试剂盒
This assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM).
No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.
No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.
Recovery
白细胞激活黏附因子(ALCAM)检测试剂盒
Matrices listed below were spiked with certain level of recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM) and the recovery rates were calculated by comparing the measured value to the expected amount of Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-101 | 95 |
| EDTA plasma(n=5) | 89-104 | 98 |
| heparin plasma(n=5) | 86-104 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activated Leukocyte Cell Adhesion Molecule (ALCAM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 82-96% | 78-104% | 83-92% |
| EDTA plasma(n=5) | 93-105% | 89-101% | 88-97% | 80-93% |
| heparin plasma(n=5) | 90-103% | 96-105% | 97-105% | 96-105% |
Stability
白细胞激活黏附因子(ALCAM)检测试剂盒
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
白细胞激活黏附因子(ALCAM)检测试剂盒
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activated Leukocyte Cell Adhesion Molecule (ALCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activated Leukocyte Cell Adhesion Molecule (ALCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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