Apa I FDRE1124

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产品价格： < 面议

产品型号：FDRE1124

品牌：Vivantis Technologies Sdn. Bhd.

公司名称：上海拜力生物科技有限公司

所在地：上海闵行

发布时间：2012-07-30

浏览次数：929 次

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产品简介

Home  Restriction Endonucleases  Fast Digest Restriction Endonucleases  Apa I
Apa I
Concentration
20u/μl 

5 ' . . . G G G C C↓C . . . 3 '
3 ' . . . C↑C C G G G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

产品详细信息

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Restriction Endonucleases

Fast Digest Restriction Endonucleases

Apa I

Concentration
20u/μl
5 ' . . . G G G C C↓C . . . 3 '
3 ' . . . C↑C C G G G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay
After 20-fold overdigestion with Apa I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Apa I for 16 hours at 37˚C.