ECACC General Cell Collection：10032309 PEO4

一般细胞收集细节

ECACC一般细胞收集：PEO4

受供于：

欧洲认证细胞培养物保藏中心（ECACC）

文化类型：

细胞系

采集：

ECACC General Collection

产品编号：

10032309

细胞系名称：

PEO 4

引文指导：

如果使用这种文化产生科学出版物，则应在出版物中引用：PEO4（ECACC 10032309）

关键词：

人卵巢癌; 雌激素受体阳性

细胞系描述：

PEO4 is an adherent cell line derived from a malignant effusion from the peritoneal

ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient

previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO4was

collected after clinical resistance developed to chemotherapy. PEO4 is tumourigenic in

immunologically-deprived CBA mice. PEO4 exhibits good growth in semi-solid medium

(agar). PEO4 is from the same patient as the PEO1 and PEO6 cell lines (ECACC

catalogue numbers 10032308 & 10032310).This cell line is one of nine from the PE

ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian

cancer, isolated from various malignant sites, and at various treatment stages) available

at ECACC which provides a model system for research into the mechanism of oestrogen

action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity

of oestrogen protagonists.

种类：

人的

原产地组织：

子房

单元格类型：

上皮细胞; 均匀多边形细胞的岛屿。

成长模式：

追随者

DNA简介：

STR-PCR数据：

釉原蛋白：X

CSF1PO：10,12

D13S317：10

D16S539：9

D5S818：11,12

D7S820：10

THO1：9.3

TPOX：9,11

vWA：15,16

核型：

对于模态染色体数目，参见Langdon SP等。（1988）癌症研究11月1日; 48（21）：

6166-72。2019/9/17

ECACC General Cell Collection

：

10032309 PEO4

生物**信息：

Unless specified

otherwise, at the European

C

ollection of Authenticated Cell Cultures

(ECACC) we routinely handle all of our cell lines at containment level 2 in accordance

with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)

All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the

responsibility of the end user to ensure that their facilities comply with biosafety

regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare

premises.

Hyperlinks to MSDS documents:

Frozen cell cultures Material Safety Data Sheet

Growing cell cultures Material Safety Data Sheet

Nucleic acids derived from cell cultures Material Safety Data Sheet

Subculture Routine:

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10 cells/cm using

0.25% trypsin or trypsin/EDTA; 5% CO ; 37°C. Doubling time approximately 36 hours.

Cultures should be media changed every 2-3 days if not splitting.

Culture Medium:

RPMI 1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum

(FBS).

Depositor:

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street

London EC1V 4AD

Originator:

Yes

Country:

UK

References:

Langdon SP et.al. (1988) Characterization and properties of nine human ovarian

adenocarcinoma cell lines. Cancer Research Nov 1; 48(21):6166-72. PMID: 3167863.

Additional Bibliography:

Langdon SP et.al. (1990) Oestrogen receptor expression and the effects of oestrogen

and tamoxifen on the growth of human ovarian carcinoma cell lines. British Journal of

Cancer Aug; 62(2):213-6. PMID: 2386737.

Patents:

None specified by Depositor

Release Conditions:

Yes - commercial organisations are required to complete the 'Cell Line Release

Authorisation for Research Use in Commercial Organisations' release conditions form.

Product Images

10032309_PEO4_Growth Profile

Note: Links open in a new window

Product Documents

10032309_PEO4_Images

Passage numbers explained

CRT Cell Line Release Authorisation for Research Use in Commercial Organisations

Note: Links open in a new window

Related Products

You might be interested in the following related products:

ECACC General Cell Collection - PEO1

ECACC General Cell Collection - PEO6

ECACC General Cell Collection - PEO14

ECACC General Cell Collection - PEO16

ECACC General Cell Collection - PEO23

ECACC General Cell Collection - TO14

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to

ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for

any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the

4 2

22019/9/17

ECACC General Cell Collection

：

10032309 PEO4

cell culture and not for any specific property of

the cell line, therefore furth

er

references should be obtained

regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections

does not guarantee the passage number stated will be the passage number received by the customer.

Culture Collections提供的文化仅供研究之用。关于细胞系的商业用途的询问涉及细胞系的保藏者。一些细胞系具有

额外的特殊释放条件，例如在供应细胞系之前需要由潜在接受者完成材料转移协议。请查看供应条款和条件以获取

更多信息。

可用格式

冷冻

生长

DNA-5μg（100ng /μl）

RNA-5μg（100ng /μl）

cDNA-20μl

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ECACC General Cell Collection：10032309 PEO4