Immortalized Human Mesothelial Cells - SV40

Immortalized Human Mesothelial Cells - SV40 T0470

永生化人间皮细胞系-SV40 上海 安徽 合肥 ABM细胞库

15000元  货期2周

	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Mesentery Tissue
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	APOBEC3, CD105, VEGF
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Description	The Immortalized Human Primary Mesothelial cells-SV40 are derived from the human mesothelium, an important structure lining human abdomen cavities to provide lubrication, transportation and movement of fluids, immune cells, cytokines and proteins. These specialized cells are critical in regulating inflammatory responses and innate immune systems to protect against infections and tumor propagation. Their roles in cancer metastasis, metabolic diseases, peritoneal dialysis and surgical adhesions make them an ideal model for cancer research, pathological and/or therapeutic study.
Procedure Overview
Propagation	Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow I medium available in ABM, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%.
Quality Control	Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
公司简介	合肥星肽生物科技有限公司作为专业的ABM中国代理商，专业经营原装进口胎牛血清，细胞因子，ELISA试剂盒，细胞，抗体，生物试剂，耗材，培养基，一抗，二抗等.ABM安徽代理的产品涉及分子生物学，细胞生物学，细菌学，遗传学，免疫学，生物化学，蛋白质学，细胞治疗，临床应用等领域。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供，中期合同保证，后期货物跟踪到*终售后的确保项目准确到位，都有相关专业人士进行维护，确保您在合肥合肥星肽生物科技有限公司公司获得***服务！
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