人星形胶质细胞 HA

货号	1800
产地	美国
缩写	HA
规格	5 x 10^5 /1ml
用途	科研
储存	液氮
运输	干冰
关键字	人星形胶质细胞 HA   人星形胶质细胞 HA  人星形胶质细胞 HA   人星形胶质细胞 HA  人星形胶质细胞 HA   人星形胶质细胞 HA
公司简介	合肥星肽生物科技有限公司作为专业的ScienCell中国代理商，专业经营实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发。合肥星肽生物科技有限公司始终致力于为客户提供可靠的研究材料以及方便快捷的服务，对购买项目的前期资料提供,中期合同保证,后期货物跟踪到*终售后的确保项目准确到位，都有相关专业人士进行维护,确保您在合肥合肥星肽生物科技有限公司公司获得***服务！
联系方式	何经理，合肥星肽生物科技有限公司，Tel：      400-8702-898，                                                  E-mail：info@qingbio.com      http://www.qingbio.com      QQ:514713116

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1、Question:

Which genes could represent good housekeeping genes for RT-qPCR analysis of these cells? Any suggestions?

Answers:

We recommend β-actin, GAPDH, LDHA, NONO, and PPIH to serve as housekeeping genes for astrocyte qRT-PCR. We also recommend using multiple housekeeping genes for data normalization. In addition, in a qRT-PCR assay, we recommend using Genomic DNA (gDNA) Control (GDC) to detect possible gDNA contamination, and Positive PCR Control (PPC) to test whether samples contain inhibitors or other factors that may negatively affect gene expression results. ScienCell offers GeneQuery? Human Astrocyte Cell Biology qPCR Array Kit for astrocyte qRT-PCR analysis.

2、Question:

WAre this cell lines？

Answers:

They are primary cells, not immortalized cell lines. thanks for your interest

3、Question:

What is the recommended maximum number of passages that the primary human astrocytes can be grown to without losing phenotype? Is there a recommended freezing protocol?

Answers:

Human Astrocytes can be subcultured for 4-5 passages. However, for primary cells, the markers such as GFAP may be lost during each passage, even though they are still astrocytes. Therefore, we recommend that customers use the cells at early passage without expanding or passaging too much. We do not recommend re-freezing the cells for future use. thanks for your interest