产品详情
  • 产品名称:Rabbit Na+-Ca2+ exchanger

  • 产品型号:p11-13
  • 产品厂商:Swant
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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简单介绍:
品牌:Swant 货号:p11-13 品名:Rabbit Na+-Ca2+ exchanger 规格:200
详情介绍:

产品介绍

名称

 Rabbit Na+ -Ca2+ exchanger 

货号

 p11-13

规格

 200ul

形式 

 Lyophilized antiserum (no preservatives). 

复溶

 with 200 µl bidistilled water.

产品描述

This antiserum was produced against canine cardiac sarcolemmal Na+ -Ca2+ exchanger (NCX1; full length isolated protein). The specific epitope was never mapped, but is likely to be primarily on the large intracellular loop. Brain, smooth muscle and kidney Na+ -Ca2+ exchanger are also recognized by the antiserum. The antiserum cross reacts with the proteins of mouse, rat, rabbit, frog, toad, guinea pig, cow and human. It does not react with NCX2 or NCX3. The antiserum can be used for immunoblots, immunoprecipitation and immunohistochemistry.  

稀释

 Immunohistochemistry; 1:5’000 - 1: 10’000 with the avidin-biotin method 

Immunoblots: 1:100 – 1: 1’000 (use milk for blocking). Primary bands at 120 kDa (full length protein), 160 kDA (some non-reduced protein even in the presence of DDT) and 70 kDa (active proteolytic fragment). 

We recommend that the optimal dilutions be determined by titration experiments. 

贮存

 For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

参考资料

 Philipson K.D. et al. (1988) Purification of the cardia Na+ -Ca2+ exchanger protein. Biochim. Biophys. Acta 945: 298-306.

 
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