产品详情
  • 产品名称:PathHunter CHO-K1 AR Protein Interaction Cell Line

  • 产品型号:93-0454C2
  • 产品厂商:discoverx
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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简单介绍:
品牌:discoverx 品名:PathHunter CHO-K1 AR Protein Interaction Cell Line Clone 货号:93-0454C2 产品规格:2 vials
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简单介绍

产品简介

  • 产品编号:93-0454C2 
  • 产品品牌:DiscoverX
  • 产品名称:PathHunter® CHO-K1 AR Protein Interaction Cell Line
  • 产品规格:2 vials
  • 简要描述
    Receptor Family
    NHR
    Coupling
    N/A
    Accession Number
    NM_000044
    Receptor Species
    Human
    Prolink™ Tag
    PK1
    Cell Background
    CHO-K1
    Agonist/Ligand
    D(-)-Norgestrel
    Cells were plated in a 384-well plate, incubated overnight at 37°C and stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® Detection kit according to the recommended protocol.

PathHunter® CHO-K1 AR Protein Interaction Cell Line

The PathHunter® NHR Protein Interaction assays monitor the interaction of activated NHRs with Steroid Receptor Co-activator Peptides (SRCPs) using Enzyme Fragment Complementation. The cells are engineered to express two complementary fragments of β-galactosidase: the smaller ProLink™ (PK) fragment is fused to the NHR target of interest, and the larger Enzyme Acceptor (EA) is fused to the SRCP. Activation of the NHR target induces interaction of the receptor with the SRCP, forcing complementation of PK and EA. Activity of the resulting fully formed β-galactosidase enzyme is detected using a chemiluminescent substrate.

Catalog No. Size Price Quantity
93-0454C2 2 vials $10,399.00
Nuclear Hormone Receptor (NHR) activation is comprised of four steps: 1) ligand binding to the NHR, 2) nuclear translocation, 3) recruitment of a Steroid Receptor Coactivator Peptide (SRCP), and 4) transcriptional activation of target DNA sequence. PathHunter products represent novel cell-based applications of the established Enzyme Fragment Complementation (EFC) technology pioneered by DiscoveRx. PathHunter NHR assays were developed using EFC with two different approches, Nuclear Translocation and Protein Interaction.

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