LOCUS Exported 3752 bp ds-DNA circular SYN 21-SEP-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3752)
TITLE Direct Submission
JOURNAL Exported Sep 21, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3752
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(151..888)
/codon_start=1
/label=Erm
/translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
LSTVTYEQVLSIFNSYLLFNGRK"
CDS 2216..2914
/codon_start=1
/label=repA
/translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
VLDAETGEIK"
promoter 3647..3665
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 3674..3751
/label=MCS
ORIGIN
1 ctagagcggc cgccaccgcg gtgggatcct ctagagtccg ctagggacct ctttagctcc
61 ttggaagctg tcagtagtat acctaataat ttatctacat tccctttagt aacgtgtaac
121 tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata atagataact
181 attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta ctttggcgtg
241 tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc gattgaccca
301 ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg tggtatggcg
361 ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt ccgctggcag
421 cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg ttcggtgaat
481 atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag acgcatggct
541 ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatac tcccaacaat
601 tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac
661 acgagtattc agttttaatt tttctgacga taagttgaat agatgactgt ctaattcaat
721 agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt tacctgttcc
781 aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt ggttgagtac
841 tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt aatcactcct
901 tcttaattac aaatttttag catctaattt aacttcaatt cctattatac aaaattttaa
961 gatactgcac tatcaacaca ctcttaagtt tgcttctaag tcttatttcc ataacttctt
1021 ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag tcagcacgaa
1081 cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta tcatttcgtt
1141 tttcttttgc gcgactctag aggatcctga taaatatgaa catgatgagt gatcgttaaa
1201 tttatactgc aatcggatgc gattattgaa taaaagatat gagagattta tctaatttct
1261 tttttcttgt aaaaaaagaa agttcttaaa ggttttatag ttttggtcgt agagcacacg
1321 gtttaacgac ttaattacga agtaaataag tctagtgtgt tagactttat gaaatctata
1381 tacgtttata tatatttatt atccgatttt ttattaaaac gtctcaaaat cgtttctgag
1441 acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg cgttatcgta
1501 gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg ttagattatg
1561 aaagccgatg actgaatgaa ataataagcg cagcgccctt ctatttcggt tggaggaggc
1621 tcaagggagt atgagggaat gaaattccct catgggtttg attttaaaaa ttgcttgcaa
1681 ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg
1741 gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg ccgagtgcca
1801 atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttaagggg tttttcaatc
1861 gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt aagtagcttt
1921 attgttgttt ttatgattac aaagtgatac actaacttta taaaattatt tgattggagt
1981 tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga caaaagagca
2041 agataaaaaa ttaacagata tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc
2101 gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag cgaaagctcg
2161 cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt atatcatggc
2221 tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact caattcctaa
2281 tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc ctttacacga
2341 tatggacgaa aaaaaagata aagatacatg gaataatagt aatattatac aaaatggaaa
2401 gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg taacaataga
2461 aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc atgttgagat
2521 acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg acgctattgc
2581 taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt ttgatattga
2641 ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac ttttagatat
2701 agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc gccttagggg
2761 agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa caaactctag
2821 cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa gttatgcaaa
2881 ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga gttatttgct
2941 gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga aagatacaga
3001 gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat tattgaataa
3061 ataaaagccc cctgacgaaa gtcgaagggg gtttttattt tggtttgatg ttgcgattaa
3121 tagcaataca attgcaataa acaaaatgat cttccttcag gttatgacca tctgtgccag
3181 ttcgtaatgt ctggtcaact ttccgactct gagaaacttc tggaatcgct agagaatttc
3241 tggaatggga ttcaggagtg gacagaacga cacggatata tagtggatgt gtcaaaacgc
3301 ataccatttt gaacgatgac ctctaataat tgttaatcat gttggttacg tatttattaa
3361 cttctcctag tattagtaat tatcatggct gtcatggcgc attaacggaa taaagggtgt
3421 gcttaaatcg ggccattttg cgtaataaga aaaaggatta attatgagcg aattgaatta
3481 ataataaggt aatagattta cattagaaaa tgaaagggga ttttatgcgt gagaatgtta
3541 cagtctatcc ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3601 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
3661 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
3721 cgaattcctg cagcccgggg gatccactag tt
//