LOCUS Exported 2845 bp ds-DNA circular SYN 28-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pUC57-Simple-gRNA backbone
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2845)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 28, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..2845
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 234..252
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_RNA 271..346
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 888..992
/gene="bla"
/label=AmpR promoter
CDS 993..1853
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2024..2612
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
181 caagctcgct tgcacgcctc tgcactcgtc ggtcccggca tccgataccc aattaatacg
241 actcactata ggtcgtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat
301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt aaagacccaa
361 tatctcgacg cattcgcgaa gtaccgatct ccaattcact ggccgtcgtt ttacaacgtc
421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
541 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
601 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
661 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
781 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
841 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
1021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
1081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
1141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
1201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
1261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
1321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
1381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
1441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
1501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
1561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
1621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
1681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
1741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
1801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
1861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
1921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
2161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
2221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
2281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
2341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
2401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
2461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
2521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
2581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
2641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
2701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
2761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
2821 ccgcgcgttg gccgattcat taatg
//