Amyloid Precursor Protein抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-20607R
英文名称Nestin
中文名称巢蛋白/神经上皮干细胞蛋白抗体
别 名ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_MOUSE; Nestin.
规格价格100ul/1280元 购买 200ul/1980元 购买 大包装/询价
说 明 书100ul 200ul
研究领域肿瘤 心血管 细胞生物 神经生物学 干细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Mouse, Rat,
Amyloid Precursor Protein抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Amyloid Precursor Protein抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Amyloid Precursor Protein抗体溶解方法:
1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):
溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;
若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。
溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)
的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。
3、抗体的分装
抗体溶解后,将其分装成 5-10支(以0.1mlAmyloid Precursor Protein抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。
另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
Amyloid Precursor Protein抗体关联产品列表:
bs-11045R No 合格 C1QL1 补体组分1q子成分样蛋白1抗体
bs-11057R No 合格 Elastin 项韧带弹性蛋白抗体
bs-1105R YES 合格 Caspase-12 半胱胺酸蛋白酶蛋白-12抗体
bs-11069R No 合格 procollagen type IIA Ⅱ型胶原α1 N端前体肽抗体
bs-11073R No 合格 CDH18 钙粘附分子18抗体
bs-11074R No 合格 CNTN4/AXCAM 轴突相关粘附分子抗体
bs-1107R No 合格 PTHLH 甲状旁腺**相关蛋白抗体
bs-11086R No 合格 合格 JAMC 连接粘附分子C抗体
bs-11093R No 合格 Myelin PLP 髓磷酯髓鞘蛋白1抗体
bs-11094R No 合格 NCAM2 神经细胞粘附分子2抗体
bs-11098R No 合格 NLGN2 神经连接蛋白2抗体
bs-11109R No 合格 PCDH12 原钙粘附蛋白12/血管内皮钙粘蛋白2抗体
bs-1110R YES 合格 合格 合格 Osterix 成骨相关转录因子抗体
bs-11112R No 合格 合格 PCDH18 原钙粘附蛋白18抗体
bs-11113R No 合格 PCDH20 原钙粘附蛋白20抗体
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