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Calponin 1抗体

Calponin 1抗体
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  • 产品名称:Calponin 1抗体
  • 产品型号:bs-0095R
  • 产品展商:Bioss
  • 产品文档:无相关文档
简单介绍
Calponin 1抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果��现货.​我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数.Calponin 1抗体可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品描述

 

Calponin 1抗体ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。


产品编号bs-0095R

英文名称Calponin 1

中文名称钙调节蛋白-1抗体

别    名Basic calponin; Calponin 1; Calponin-1; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin1; Calponins basic; CNN 1; CNN1; Sm Calp; SMCC; CNN1_HUMAN; Calponin H1; Calponin1; Sm Calp; smooth muscle.  

JournalPMIDIFApplicationMolecular Endocrinology (2014)253214134.7460IHC-PTissue Engineering (2014)244176864.0650IHC-F

规格价格50ul/780元 购买    100ul/1280元 购买    200ul/1980元 购买    大包装/询价

说 明 书50ul  100ul  200ul

研究领域心血管  信号转导  干细胞  细胞骨架  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,

Calponin 1抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

 


177kDa

细胞定位细胞浆

   Lyophilized or Liquid

   1mg/1ml

KLH conjugated synthetic peptide derived from human Nestin

   IgG

纯化方法affinity purified by Protein A

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

Calponin 1抗体产品介绍background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

Calponin 1抗体溶解方法:

1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml

2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):

溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;

若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。

溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)

的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。

3、抗体的分装

抗体溶解后,将其分装成 5-10支(以0.1mlCalponin 1抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。

另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。

后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。

我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。

Calponin 1抗体关联产品列表:

bs-1050R YES 合格 合格 Connexin-40 间隙连接蛋白40抗体  
bs-1052R No 合格 NAP1L1 核小体组装蛋白1抗体
bs-10539R No 合格 合格 TTF1 甲状腺核转录因子-1抗体
bs-10540R No 合格 IL-1 Alpha Propeptide 白介素1α前肽/IL-1α前肽抗体
bs-10549R No 合格 CD55 衰变加速因子CD55抗体
bs-10556R No 合格 SLC27A1 长链脂肪酸转运蛋白1抗体
bs-10557R No 合格 GOT2 谷草转氨酶2抗体
bs-10558R No 合格 beta Amyloid 1-16 β淀粉样肽1-16/Aβ1-16抗体
bs-10559R No 合格 beta Amyloid 1-28 β淀粉样肽1-28/β-Amyloid 1-28抗体
bs-1056R YES 合格 5HT2A Receptor 5-羟色胺受体2A抗体
bs-10574R No 合格 Centrin 1 中心体蛋白1抗体
bs-10578R No 合格 WASF3 Verprolin同源结构域包含蛋白3抗体
bs-10581R No 合格 合格 MMP13 基质金属蛋白酶13抗体
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