VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothellial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase(flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant Rat VEGF165 is a 38.5 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
Source: E.coli
Synonyms: Vascular Endothelial Growth Factor, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF-A
AA Sequence (monomer): MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/ml.
