- Description
- Data Sheet
A fully validated, highly specific affinity purified mouse monoclonal antibody to skeletal muscle SERCA1
Background: SERCA1 is the Ca2+-pump (109kDa) located in the sarcoplasmic reticulum (SR) of fast-twitch skeletal muscle. The enzyme transports 2 Ca2+ from the cytoplasm into the SR using the hydrolysis of 1 molecule of ATP to power the reaction. This terminates muscle contraction and promotes muscle relaxation. Mutations in the SERCA1 gene (ATP2A1) are observed in some patients with Brody disease, an exercise induced impairment in muscle relaxtion, stiffness and cramps. The monoclonal antibody B/4H3 was produced by immunizing mice with purified SERCA1: the antibody inhibits SERCA1 activity upon binding
Tested Applications: Western Blot (1:5000 dilution), IHC Microscopy (1:200 dilution). Species recognition: Antibody tested on Rat and rabbit tissue; immunogenic peptide aligns with Mammalian species, Avian, Amphibian and Fish
| Description | Affinity purified Mouse monoclonal antibody to SERCA1 |
| Immunogen | Purified SERCA1a. Epitope was mapped to residues 662-666 |
| Isotype | IgG |
| Purification | Protein A affinity purified |
| Species reactivity | Antibody tested on Rat and Rabbit tissue, but immunogenic peptide aligns with Mammalian species, Avian, Amphibian and Fish |
| Tested applications | WB |
| Recommended dilution | WB 1:5000 |
| Optimization | Optimal dilutions to be determined by end user |
| Storage | Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles. |
| Storage | Lyophilised: 4 °C. Reconstituted:-80 °C |
| Datasheet | A010-22AP
 |
| MSDS | A010-22AP
|
| Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |
