- Description
- Data Sheet
A fully validated, highly specific polyclonal antiserum to Serine-16 phosphorylated forms of phospholamban
Background: Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration. Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent, and cGMP-dependent (Ser-16) and Ca2+/CaM-dependent (Thr-17) protein kinases in response to β-adrenergic stimulation. Akt has also been shown to phosphorylate Thr-17. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure
Tested applications: Western Blot (1:5000 dilution), ELISA. Species recognition: all mammalian species, when phosphorylated on Ser-16.
| Description | Lyophilised Rabbit polyclonal anti-serum (A010-12) containing IgG antibody specific for Ser-16 phosphorylated forms of PLB (Drago & Colyer, 1994). |
| Immunogen | Phosphopeptide comprising residues 9-19-Y (residues R9SAIRRAS(PO3H2)TIE19Y) conjugated to KLH. |
| Isotype | IgG |
| Purification | Raw serum |
| Species reactivity | The antibody recognises mono and oligomeric phospholamban when phosphorylated on serine-16 by PKA. Binding of the antibody to its target epitope is blocked in the presence of a phosphopeptide containing the PLB Phospho Ser-16 epitope. Antibody affinity is reduced in circumstances of dual phosphorylation of Ser-16 and Thr-17. |
| Tested applications | WB, ELISA and IHC microscopy |
| Recommended dilution | WB 1:5000 |
| Optimization | Optimal dilutions to be determined by end user |
| Storage | Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 25 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles. |
| Storage | Lyophilised: 4 °C Reconstituted:-80 °C |
| Datasheet | A010-12
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| MSDS | A010-12
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| Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |
