lucigen 60442-2 (SOLOs) 60080-2 (DUOs) 24 rxns 感受态细胞
产品简介：北京中北林格供应lucigen 60442-2 (SOLOs) 60080-2 (DUOs) 24 rxns 感受态细胞，中北林格是lucigen总代理，提供lucigen产品货号报价查询。其他包括epicentre总代理，cellscript总代理，Biosearch technologies总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p
产品名称:
OverExpress™ C41 (DE3) Chemically Competent Cells
货号:60442-2
规格:24 rxns (SOLOs)
存储温度:-80º C
产品名称:
E. cloni® 10G SUPREME Electrocompetent Cells
货号:60080-2
规格:24 rxns (DUOs)
存储温度:-80º C
lucigen 60442-2 (SOLOs) 60080-2 (DUOs) 24 rxns 感受态细胞
lucigen 60442-2 24 rxns (SOLOs) 化学感受态细胞 产品说明书
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Express genes cloned into any T7 vector with these BL21(DE3) derivatives
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Effective in expressing toxic & membrane proteins
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Cited in over 350 research articles
E. coli BL21(DE3) strains, like Lucigen’s E. cloni® EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e.g., pET or Lucigen’s pSMART®-cDNA vectors). However, in some cases expression is minimal or not detectable because the recombinant protein, when expressed, is deleterious or lethal to these standard BL21 strains. Examples of such toxic proteins include many membrane proteins, some cytoplasmic proteins, and nucleases. Unfortunately, successful expression of one or more toxic proteins is often important to the experimental goal.
Lucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications.
像Lucigen的E. cloni®EXPRESS感受态细胞这样的大肠杆菌 BL21（DE3）菌株 可可靠地表达克隆到T7表达载体（例如pET或Lucigen的pSMART®-cDNA载体）中的许多基因。但是，在某些情况下，表达很少或无法检测到，因为重组蛋白表达时对这些标准BL21菌株有害或致死。这种有毒蛋白质的例子包括许多膜蛋白，一些胞质蛋白和核酸酶。不幸的是，一种或多种毒性蛋白的成功表达通常对实验目标很重要。
Lucigen的过表达电感受态细胞和化学感受态细胞是 大肠杆菌 菌株，可有效表达来自各种生物体（包括真**，酵母，植物，病毒和哺乳动物）的有毒蛋白质。这些新菌株在表达有毒蛋白质方面的有效性已在350多个出版物中得到验证。
图例说明：
Figure 1. Green Fluorescent Protein (top) or Red Fluorescent Protein (bottom) expressed from a T7 promoter construct that was transformed into C41, BL21, or C43 competent cells spread on IPTG plates to induce protein expression.
Figure 2. Comparison of OverExpress C41(DE3) and C43(DE3) cells with the parental strain BL21(DE3) in transformation and expression of heterologous proteins.**
Contact your local distributor for pricing
常问问题：我应该使用哪种过表达细胞株？
It is difficult to predict which of the four OverExpress strains – C41(DE3), C43(DE3), C41(DE3)pLysS, or C43(DE3)pLysS – will work best in expressing a given protein. We recommend initially using the OverExpress ComboPack™ which contains 3 reactions each of the four OverExpress competent cell strains, to determine which one is best for your application. The OverExpress strains are available as electrocompetent or chemically competent cells.
Because there are no intrinsic antibiotic resistances (or plasmids) in either C41(DE3) or C43(DE3), the strains can be differentiated from each other and from BL21(DE3) by transformation with a strain verification vector, pAVD10. pAVD10 contains the uncF gene (encoding the beta-subunit of E. coli ATPase) under the control of the T7 promoter. This plasmid is lethal to BL21(DE3) and to induced C41(DE3), but it is tolerated by C43(DE3) regardless of induction. pAVD10 is provided with OverExpress Cells.
lucigen 60442-2 (SOLOs) 60080-2 (DUOs) 24 rxns 感受态细胞
lucigen 60080-2 24 rxns (DUOs) 电感受态细胞 产品说明书
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Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
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Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
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Available in a range of high transformation efficiencies (5 × 109 to 4 × 1010 cfu/µg).
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Convenient packaging options.
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.
Choice of strain:
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E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
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E. cloni 10GF′Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.
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