lucigen 30059-0 30059-1 30059-2 CloneID™ 1X Colony PCR Master Mix
产品简介：北京中北林格供应lucigen 30059-0 30059-1 30059-2 CloneID™ 1X Colony PCR Master Mix，中北林格是lucigen总代理，提供lucigen产品货号报价查询。其他包括cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
产品名称:CloneID™ 1X Colony PCR Master Mix
货号:30059-2
规格:500 rxns
存储温度:-80º C
品牌：lucigen / epicentre</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
lucigen 30059-0 30059-1 30059-2 CloneID™ 1X Colony PCR Master Mix
lucigen 30059-0 30059-1 30059-2 CloneID™1X菌落PCR预混液
产品说明书
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Outstanding reliability and reproducibility in Colony PCR
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Ideal for PCR genotyping
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Convenient 1X Master Mix: just add a colony, primers, and cycle.
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High thermostability and optimized formulation for 98C denaturation to handle the toughest targets.
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Contains Green tracking dye for direct gel loading
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菌落PCR的出色可靠性和可重复性
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PCR基因分型的理想选择
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便捷的1X预混液：只需添加菌落，引物和循环即可。
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高热稳定性和针对98C变性的优化配方，可应对zui困难的目标。
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包含绿色跟踪染料，可直接上胶
Finally, a PCR enzyme specifically formulated for Colony PCR.
Reliable performance from colonies. Bypass all cell lysis and sample prep without worrying about failed reactions. Outstanding reproducibility to get your amplicon the first time. (Figure 1).
Extreme thermostability. Cycle at 98°C to lyse cells and denature the toughest templates. The added PCR enhancer improves amplification and thermostability to give you the best PCR product possible.
Tough templates are no match: GC-rich DNA can make colony PCR that much more difficult – and CloneID can handle it with ease. Clean and robust amplification on targets where other enzymes fall short (Figure 2)
The easiest workflow. CloneID is not only a 1X master mix that only requires a colony swipe and primers, but it also contains a Green tracking dye so you can directly load your PCR product onto a gel.
专门为菌落PCR配制的PCR酶。
殖民地的可靠表现。 绕过所有细胞裂解和样品制备过程，而不必担心失败的反应。出色的可重复性，可在第1时间获得您的扩增子。（图1）。
极高的热稳定性。 在98°C循环以裂解细胞并使zui坚韧的模板变性。添加的PCR增强剂可改善扩增和热稳定性，从而为您提供zui佳的PCR产物。
模板 很难匹配：富含GC的DNA会使菌落PCR变得更加困难-CloneID可以轻松处理。在其他酶不足的靶标上进行干净而有力的扩增（图2）
zui简单的工作流程。 CloneID不仅是一种1X预混液，只需要菌落轻扫和引物，它还包含绿色跟踪染料，因此您可以将PCR产物直接加载到凝胶上。
lucigen 30059-0 30059-1 30059-2 CloneID™ 1X Colony PCR Master Mix 图例说明
Figure 1. CloneID™ 1X Colony PCR Master Mix was used on 96 unique colonies from a petri dish containing a library sample. Success rate was 97% (93 out of 96) for amplification.
图1. CloneID™1X菌落PCR预混液用于培养皿中含有培养皿样品的96个独特菌落。扩增成功率为97％（96个中的93个）。
Figure 2. CloneID™ 1X Colony PCR Master Mix is compared with other PCR products (CloneID™, Platinum® PCR Supermix, OneTaq®, KAPA2G) claiming strong Colony PCR performance. The 12 targets are genes fromCellulomonas fimi, a species whose genome is 75% GC, which were cloned into a standard plasmid.
图2.将CloneID™1X Colony PCR Master Mix与声称具有强大Colony PCR性能的其他PCR产品（CloneID™，Platinum®PCR Supermix，OneTaq®，KAPA2G）进行比较。这12个靶标是来自Cellulomonas fimi的基因，该基因组为75％GC，被克隆到标准质粒中。
Contact your local distributor for pricing
ORDER INFORMATION订单信息
CloneID™ 1X Colony PCR Master Mix is provided in a 1X Master Mix format that withstands 98°C denaturation. All required buffer components, enzyme, Mg++ and dNTP’s are all present in optimized concentrations. The product also contains Green tracking dye for convenient gel loading. Only a small amount of whole cells from a colony and primers are required to set up the reaction. Please see the user manual for composition information. Standard reaction size is 25 µL.
CloneID™1X Colony PCR Master Mix以1X Master Mix格式提供，可承受98°C变性。所有必需的缓冲液成分，酶，Mg ++和dNTP都以优化的浓度存在。该产品还包含绿色跟踪染料，可方便地加载凝胶。仅需要来自菌落和引物的少量全细胞来建立反应。请参阅用户手册以获取成分信息。标准反应大小为25 µL。
该产品主要用于 大肠杆菌 菌落或纯化的DNA模板。CloneID尚未针对多种生物进行测试。
This product is primarily intended for E. coli colonies or purified DNA templates. CloneID has not been tested for diverse organisms.
对此产品有兴趣，可联系中北林格获取更多详细信息。
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