epicentre SF81015 1.5 ml SA85025 2.5 ml
产品简介：北京中北林格供应epicentre SF81015 1.5 ml SA85025 2.5 ml，中北林格是lucigen总代理，提供lucigen产品货号报价查询。其他包括cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
产品名称:
SequiTherm EXCEL™ II DNA Polymerase
epicentre SF81015 1.5 ml SA85025 2.5 ml
货号:SED52100
规格:100 Rxns
存储温度:-20℃
品牌：lucigen / epicentre</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
epicentre SF81015 1.5 ml SA85025 2.5 ml 产品描述
Applications
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Automated sequencing of all DNA templates, including those containing difficult-to-sequence regions that cannot be resolved with other sequencing kits (Figs. 1-4).
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对所有DNA模板进行自动测序，包括那些含有难以用其他测序试剂盒解析的难以测序区域的DNA模板（图1-4）。
SequiTherm EXCEL II™ Sequencing Kits for automated sequencers use the SequiTherm EXCEL II Polymerase, which improves sequencing performance with increased signal intensities and the ability to sequence through GC-rich regions. The SequiTherm EXCEL II DNA Sequencing Kits incorporate the use of betaine,* which enables sequencing of a broad range of templates, including those containing regions that are very difficult to sequence. Protocols are provided for both cycle and high-temperature isothermal (noncycle) sequencing.
用于自动测序仪的SequiTherm EXCEL II™测序试剂盒使用SequiTherm EXCEL II聚合酶，该酶可提高测序性能，同时增加信号强度，并能够通过富含GC的区域进行测序。SequiTherm EXCEL II DNA测序试剂盒结合了甜菜碱的使用，*可以对多种模板进行测序，包括那些很难测序的区域。提供了用于循环和高温等温（非循环）测序的方案。
The kits have been optimized for use with the following DNA sequencers: LI-COR™ 4000 and 4200 series Sequencers, NEN™ Global IR2 DNA Sequencers, ALF™ DNA Sequencers, and ABI PRISM™ DNA Sequencers using dye primer technology. Using primers labeled with standard sequencing dyes, the SequiTherm EXCEL II kits may also be used with ABI PRISM 373 and 377 series sequencers. The kits are not compatible with ABI capillary sequencers (ABI 310, 3100, or 3700 series) or with BigDye™ technology.
该试剂盒经过优化，可与以下DNA测序仪配合使用：LI-COR™4000和4200系列测序仪，NEN™Global IR 2 DNA测序仪，ALF™DNA测序仪和使用染料引物技术的ABI PRISM™DNA测序仪。使用标有标准测序染料的引物，SequiTherm EXCEL II试剂盒还可与ABI PRISM 373和377系列测序仪一起使用。该套件与ABI毛细管测序仪（ABI 310、3100或3700系列）或BigDye™技术不兼容。
Two different kits are available for use with LI-COR™ and NEN™ sequencing equipment. The first kit (Cat. No. SE9101LC) contains Termination Mixes that give optimal read lengths up to about 1,000 bases and is designed for use on 25- to 41-cm gels. The second kit (Cat. No. SE9202LC) provides enhanced signal intensities in the resolution range of 1,000-1,400 bases and is ideal for users interested in obtaining the longest possible reads in conjunction with 66-cm gels, version 4.x of the BaseImagIR™ software, and all versions of e-Seq™ software.
有两种不同的套件可与LI-COR™和NEN™测序设备一起使用。第1个试剂盒（目录号SE9101LC）包含终止混合物，可提供长达1000个碱基的zui佳读取长度，并设计用于25至41厘米的凝胶。**个试剂盒（目录号SE9202LC）在1,000-1,400碱基的分辨率范围内提供增强的信号强度，对于希望与66-cm凝胶（版本4.x的BaseImagIR）一起获得尽可能长的读数的用户来说是理想的选择™软件以及所有版本的e-Seq™软件。
epicentre SF81015 1.5 ml SA85025 2.5 ml 好处
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序列模板包含发夹结构（图3），高GC或AT区域（图1），链间重退火区域或核苷酸重复序列（图4）。
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降低的背景信号可产生更清晰，更少歧义的序列数据（图2）。
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与LI-COR，NEN和ALF DNA测序仪（染料引物和内部标签）以及ABI PRISM自动测序仪（染料引物）兼容的试剂盒。
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可选的等温方案，可对循环测序不利的模板进行测序。终止混合物包含7-脱氮-dGTP，以减少凝胶电泳过程中的条带压缩。
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Sequence templates containing hairpin structures (Fig. 3), regions of high GC or AT (Fig. 1) content, areas of interstrand reannealing, or nucleotide repeats (Fig. 4).
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Reduced background signal results in clearer, less ambiguous sequence data (Fig. 2).
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Kits compatible with LI-COR, NEN, and ALF DNA sequencers (both dye-primer and internal label) and with ABI PRISM automated sequencers (dye-primer).
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Optional isothermal protocol to sequence templates that are recalcitrant to cycle sequencing. Termination Mixes contain 7-deaza-dGTP to reduce band compression during gel electrophoresis.
epicentre SF81015 1.5 ml SA85025 2.5 ml 图例说明：
Figure 1. Sequencing of a difficult template. An AT rich template was sequenced using a standard cycle sequencing kit (A) and the SequiTherm EXCEL™ II Kit-LC (B). As shown, several strong stops were eliminated and sequence was easily read using the SequiTherm EXCEL II Kit-LC. Samples were loaded in the order G,A,T,C. Data were visualized on a LI-COR® Model 4000 DNA Sequencer.
Figure 2. The SequiTherm EXCEL™ II Kit-LC greatly reduces nonspecific background. A plasmid DNA template was sequenced with a standard cycle sequencing kit (A) and with the SequiTherm EXCEL II Kit-LC (B). Background signal is greatly reduced using the SequiTherm EXCEL II Kit-LC. Samples were loaded in the order G,A,T,C. Data were visualized on a LI-COR® Model 4000 DNA Sequencer.
Figure 3. SequiTherm EXCEL II™ DNA Sequencing Kits provide complete, clear sequencing data when other methods fail. A supercoiled plasmid containing a putative 24-bp hairpin loop was successfully sequenced with the SequiTherm EXCEL II DNA Sequencing Kit-LC using the standard cyclesequencing protocol and IRD800-labeled primer. Data were visualized on a NEN® IR2 Model 4000L DNA Sequencer (Panel A). This same region was not resolved using the ABI BigDye™ Terminator Cycle Sequencing Kit. Data were visualized on an ABI PRISM® 310 Genetic Analyzer (Panel B).
Figure 4. Sequencing through highly problematic repeat regions. Supercoiled plasmids containing GA-dinucleotide repeats (A), GGCtrinucleotide repeats (B), or GAAA-tetranucleotide repeats (C) were successfully sequenced with the SequiTherm EXCEL™ II DNA Sequencing Kit using the standard cycle sequencing protocol and dye-labeled primers.
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