ACM04037 AmpliCap-Max™ T7 High Yield Message Maker Kit

• Generate 5´-capped RNA with a standard cap, from DNA cloned downstream of a T7 or T3 RNA Polymerase promoter, for in vitro translation and microinjection.
• Functional studies of heterogenous nuclear RNAs and viral RNAs.

The AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield Message Maker Kits are specially formulated to produce the highest yield of 5´-capped RNA (m⁷Gp₃G cap analog) from an in vitro transcription reaction in the shortest reaction time.

Benefits

• Yield up to 60 µg of RNA from 1 µg of control templates in a 20-µl reaction in 30 minutes.
• Capping efficiencies up to 80%.
• An optimized m⁷G[5´]ppp[5´]G Cap/NTP PreMix is provided for ease of use and highest yields of capped RNA transcripts.
• A separate vial of GTP is provided for efficient production of long, 5´-capped RNA.
• Formulated to use high concentrations of NTPs that are inhibitory to conventional in vitro transcription kits.
• RNase inhibitor included.

Notes:

1. Use an AmpliCap™ SP6 High Yield Message Maker Kit to produce highest yields of RNA with a standard m⁷Gp₃G cap using templates linked to an SP6 promoter.
2. If the capped RNA is to be used for translation studies, translational efficiencies can be much higher if the mRNA is also polyadenylated, especially if the mRNA is intended for use in vivo following transfection or microinjection. RNA can be easily and efficiently polyadenylated using EPICENTRE's Poly(A) Polymerase Tailing Kit.
3. Use the AmpliScribe™ T7-Flash™ and the AmpliScribe SP6 High Yield Transcription Kit to produce the highest yields of uncapped RNA.

应用领域

• 从克隆到T7或T3 RNA聚合酶启动子下游的DNA中生成带有标准盖的5´帽RNA，用于  体外  翻译和显微注射。
• 异质核RNA和病毒RNA的功能研究。

AmpliCap-Max™T7和AmpliCap-Max™T3高产量消息制作试剂盒经过特殊配制，可以 在zui短的反应时间内通过体外转录反应生产出zui高产量的5´加帽RNA（m ⁷ Gp ₃ G cap类似物）  时间。

好处

• 在30分钟内以20 µl的反应量从1 µg的对照模板中产生多达60 µg的RNA。
• 封盖效率高达80％。
• 提供了优化的m ⁷ G [5´] ppp [5´] G Cap / NTP PreMix，可简化使用并获得zui高产量的带帽RNA转录本。
• 提供了一个单独的GTP小瓶，可有效生产5'端长的RNA。
• 配制为使用抑制常规体外  转录试剂盒的高浓度NTP  。
• 包括RNase抑制剂。

笔记：

1. 使用AmpliCap™SP6高产量消息产生试剂盒，使用与SP6启动子链接的模板，可以用标准的m ⁷ Gp ₃ G瓶盖产生*高产量的RNA 。
2. 如果将带帽RNA用于翻译研究，则如果mRNA也被聚腺苷酸化，则翻译效率会更高，特别是如果打算在 转染或显微注射后将mRNA用于 体内时。使用EPICENTRE的Poly（A）聚合酶拖尾试剂盒，可以轻松高效地对RNA进行聚腺苷酸化。
3. 使用AmpliScribe™T7- Flash ™和AmpliScribe SP6高产转录试剂盒可产生*高产量的无盖RNA。