lucigen epicentre Lambda Exonuclease LE035H LE032K

• Single-strand conformation polymorphism (SSCP) analysis of PCR products.^1,2
• PCR产物的单链构象多态性（SSCP）分析。^1,2
• 从PCR产品生成ssDNA测序模板。
• Generation of ssDNA sequencing template from PCR products.

Lambda Exonuclease is a highly processive 5´→3´ exodeoxyribonuclease that selectively digests the 5´ phosphorylated strand of dsDNA. The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA. The enzyme has reduced activity against nicked DNA and against ssDNA and gapped DNA.³

^{Lambda外切核酸酶是一种高度加工性的5´→3´外脱氧核糖核酸酶，可选择性消化dsDNA的5´磷酸化链。优选的底物是平末端的5'-磷酸化dsDNA。该酶对带切口的DNA以及对ssDNA和缺口DNA的活性降低。3}

Unit Definition: One unit of Lambda Exonuclease will produce 10 nmol of acid-soluble deoxyribonucleotides from dsDNA template in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer.

单位定义：一个单位的Lambda核酸外切酶将在1X Lambda核酸外切酶反应缓冲液中于37°C在30分钟内从dsDNA模板产生10 nmol的酸溶性脱氧核糖核苷酸。

质量控制： Lambda核酸外切酶经过功能测试，以确保使用5′-磷酸化引物产生的PCR产物完全且优先降解。使用含有5´-OH末端的引物制备的PCR产物不会被消化。经测试，λ外切核酸酶不含污染性核酸内切酶活性。

Quality Control: Lambda Exonuclease is function-tested to ensure complete and preferential degradation of PCR products produced using 5´-phosphorylated primers. PCR products made using primers containing 5´-OH ends are not digested. Lambda Exonuclease is tested to be free of contaminating endonuclease activities.

References

1. Schwieger, F. and Tebbe, C.C. (1998) App. and Environ. Microb. 64, 4870.
2. Schwieger, F. and Tebbe, C.C. (2000) App. and Environ. Microb. 66, 3556.
3. Mitsis, P.G. and Kwagh, J.G. (1999) Nucleic Acids Res. 27, 3057.