Colony Fast-Screen™ Kit ( Restriction Screen) 200 Screens FS0472H

• Identification of desired clones (size and orientation) from a cloning experiment or in a library.
• 从克隆实验或文库中鉴定所需克隆（大小和方向）。
• 释放DNA进行限制性分析。
• Releasing DNA for restriction analysis.

The Colony Fast-Screen™ Kit (Restriction Screen) provides an easy and rapid method to screen the size and orientation of cloned DNA by restriction digests, without overnight cultures or plasmid minipreps (Fig. 1). The Colony Fast-Screen Kit Restricti-Lyse™ solution lyses cells in an environment which is not inhibitory to restriction enzyme activity. Thus, the kit enables the use of any restriction enzyme for restriction analysis. E. coli recombinant colonies can be screened in 25 minutes using the standard protocol (Fig. 2), or in as little as 10 minutes with the accelerated protocol (Fig. 3).

Colony Fast-Screen™试剂盒（限制性酶切）提供了一种简便，快速的方法，可通过限制性酶切来筛选克隆的DNA的大小和方向，而无需过夜培养或质粒小量制备（图1）。Colony快速筛选试剂盒Restricti-Lyse™解决方案可在不抑制限制性酶活性的环境中裂解细胞。因此，该试剂盒可将任何限制酶用于限制分析。  可以使用标准方案在25分钟内筛选出大肠杆菌重组菌落（图2），或者使用加速方案在短短10分钟内筛选出大肠杆菌重组菌落（图3）。

Benefits

• Perform restriction analysis on recombinant clone containing E. coli colonies in 10-25 minutes without the need for overnight cultures and plasmid preps.
• The Restriction Screen solution does not inhibit restriction enzyme activity.
• Amenable to high-throughput applications.
• Can detect inserts as small as 100 bp.
• 无需过夜培养和质粒制备，即可在10-25分钟内对含有大肠杆菌 菌落的重组克隆进行限制性分析  。
• 限制性内切酶溶液不抑制限制性内切酶活性。
• 适用于高通量应用。
• 可以检测到小至100 bp的插入片段。

Notes:

• The accelerated protocol involves heating the Restricti-Lyse™ Solution for 10 seconds instead of 60 seconds and incubation of the restriction digest for 5 minutes instead of 15 minutes. Restriction bands stain less intensely than when using the standard protocol, but in most cases yield a clearly discernible pattern.
• We do not recommend using the Colony Fast-Screen Kit (Restriction Screen) with single-copy-number clones.
• 加速方案包括将Restricti-Lyse™溶液加热10秒而不是60秒，并且将限制性酶切消化液孵育5分钟而不是15分钟。与使用标准协议时相比，限制带的染色强度较小，但在大多数情况下会产生清晰可辨的图案。
• 我们不建议对单拷贝数克隆使用菌落快速筛选试剂盒（限制筛选）。