epicentre SC04350 SR04350 Inhibitor Removal Resin Spin Columns
产品简介：北京中北林格供应epicentre SC04350 SR04350 Inhibitor Removal Resin Spin Columns，中北林格是epicentre总代理，提供epicentre产品货号报价查询。其他包括lucigen总代理，cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p
产品名称:Inhibitor Removal Resin Spin Columns
货号:SC04350 SR04350
规格:55 ml
存储温度:Room Temperature
品牌：lucigen / epicentre</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p
epicentre SC04350 SR04350 Inhibitor Removal Resin Spin Columns产品描述
应用领域
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从土壤和沉积物中提取可用于PCR的DNA，以分析各种生物。
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Extraction of PCR-ready DNA from soil and sediment for analysis of diverse organisms.
SoilMaster™DNA提取试剂盒提供了从各种环境样品中回收PCR就绪DNA所需的所有试剂。该试剂盒利用了热洗涤剂溶解过程以及简单的色谱步骤，可去除已知与土壤和沉积物样品中的DNA共同提取的有机抑制剂，例如腐殖酸和黄腐酸。与结合了磁珠敲打或涡旋混合的其他土壤DNA试剂盒相比，SoilMaster试剂盒可分离更大，更完整的DNA。1提取的DNA已准备好进行PCR，可用于扩增xi菌，植物或真jun模板（图2）。
The SoilMaster™ DNA Extraction Kit provides all the reagents necessary to recover PCR-ready DNA from a variety of environmental samples. The kit utilizes a hot detergent-lysis process combined with a simple chromatography step that removes organic inhibitors, such as humic and fulvic acids, that are known to coextract with DNA from soil and sediment samples. The SoilMaster Kit isolates larger and more intact DNA than other soil DNA kits incorporating bead-beating or vortex mixing.1 The extracted DNA is PCR-ready, and can be used to amplify bacterial, plant, or fungal templates (Fig. 2).
��势说明
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在不到45分钟的时间内提取可用于PCR的土壤DNA。
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Extract PCR-ready soil DNA in less than 45 minutes.
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无需专用设备或打珠。
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No need for dedicated equipment or bead-beating.
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更大，更高分子量的DNA回收率。
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Larger, higher-molecular-weight DNA recovery.
epicentre SC04350 SR04350 Inhibitor Removal Resin Spin Columns 图例说明
图1. SoilMaster™DNA提取试剂盒可从堆肥土壤样品中提取高分子量完整DNA。M泳道，kb DNA阶梯；第1泳道，用SoilMaster试剂盒提取的土壤DNA；第2和3道，使用其他土壤试剂盒纯化的DNA。
Figure 1. The SoilMaster™ DNA Extraction Kit extracts high molecular weight intact DNA from compost soil sample. Lane M, kb DNA ladder; Lane 1, soil DNA extracted with the SoilMaster Kit; Lanes 2 and 3, DNA purified using other soil kits.
图2.提取的土壤DNA的FailSafe™PCR扩增。从三种不同的土壤类型（包括洞穴沉积物（图A），森林土壤（图B）和沼泽土壤（图C））中提取DNA。使用以下引物扩增提取的土壤DNA：泳道1和2，针对16S核糖体RNA基因的共有细jun引物；3道，真jun，原生生物和绿藻引物；泳道4，植物引物NS3 / NS4；泳道5，高GC革兰氏阳性细jun引物；泳道6，芽孢杆菌引物；泳道M，100 bp DNA阶梯。
Figure 2. FailSafe™ PCR amplification of extracted soil DNA. DNA was extracted from three distinct soil types including cave sediment (Panel A), forest soil (Panel B), and marsh soil (Panel C). The extracted soil DNA was amplified using the following primers: Lanes 1 and 2, consensus bacterial primers to the 16S ribosomal RNA gene; lane 3, fungi, protist, and green algae primers; lane 4, plant primers NS3/NS4; lane 5, high-GC Gram-positive bacterial primers; lane 6, Bacillus primers; Lane M, 100-bp DNA ladder.
epicentre SC04350 SR04350 Inhibitor Removal Resin Spin Columns 货号详情
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