epicentre MOE51024 mRNA-ONLY™ Eukaryotic mRNA Isolation Kit

• 快速，容易地分离出基本上不含污染性rRNA的真核mRNA，用于基因表达分析，核糖核酸酶保护试验，RT-PCR，Northern印迹或其他操作。
• Quick and easy isolation of eukaryotic mRNA that is substantially free of contaminating rRNA, for gene expression analysis, ribonuclease protection assays, RT-PCR, Northern blots, or other procedures.
• 简化了高质量cDNA文库的生成。
• Simplified generation of high-quality cDNA libraries.

传统上，通过洗脱从固定的oligo（dT）或oligo（dU）底物中分离出3′-聚腺苷酸化的真核mRNA。mRNA-ONLY™真核mRNA分离试剂盒*提供了一种简单有效的方法，可在不到一个小时的时间内分离出基本上不含核糖体RNA（rRNA）的真核mRNA，并且不会与寡核苷酸（dT）结合。

Eukaryotic mRNA that is 3´-polyadenylated has traditionally been isolated by elution from immobilized oligo(dT) or oligo(dU) substrates. The mRNA-ONLY™ Eukaryotic mRNA Isolation Kit* provides a simple and effective method for isolating eukaryotic mRNA that is substantially free of ribosomal RNA (rRNA), in less than an hour, without binding to an oligo(dT) substrate.

仅mRNA试剂盒包括EPICENTRE的Terminator™* 5´-磷酸盐依赖性核酸外切酶，一种可消化的5´→3´核酸外切酶，可消化具有5´单磷酸的RNA。该酶不能消化具有5´三磷酸，5´帽（存在于大多数真核mRNA中）或5´-羟基的RNA。这些酶的特性使终止子核酸外切酶成为通过选择性消化rRNA（具有5´单磷酸）的真核总RNA制备物中富含mRNA的样品的理想选择。因此，仅mRNA真核mRNA分离试剂盒可用于分离基本不含5.8S，18S和28S rRNA的真核mRNA。终止子核酸外切酶消化不能去除转移RNA（tRNA）和5S rRNA。

The mRNA-ONLY Kit includes EPICENTRE's Terminator™* 5´-Phosphate-Dependent Exonuclease, a processive 5´→3´ exonuclease that digests RNA having a 5´ monophosphate. The enzyme does not digest RNA having a 5´ triphosphate, a 5´ cap (present on most eukaryotic mRNAs), or a 5´-hydroxyl group. These enzymatic properties make Terminator Exonuclease ideal for producing mRNA-enriched samples from eukaryotic total RNA preparations by selectively digesting the rRNAs (which have a 5´ monophosphate). Thus, the mRNA-ONLY Eukaryotic mRNA Isolation Kit can be used to isolate eukaryotic mRNA substantially free of 5.8S, 18S, and 28S rRNA. Transfer RNA (tRNA), and 5S rRNA are not removed by Terminator Exonuclease digestion.

优势说明

• 不需要寡聚（dT）结合步骤，可将因结合效率低下而导致的mRNA损失降至*低。
• o oligo(dT)-binding step is required, minimizing losses of mRNA due to inefficient binding.
  
• 仅mRNA的真核mRNA分离试剂盒可用于同时从原核和真核细胞制备mRNA。
• The mRNA-ONLY Eukaryotic mRNA Isolation Kit can be used to simultaneously prepare mRNA from both prokaryotic and eukaryotic cells.