lucigen MTE0970 TE Buffer
产品简介：北京中北林格供应lucigen MTE0970 TE Buffer，中北林格是lucigen总代理，提供lucigen产品货号报价查询。其他品牌包括cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
lucigen MTE0970 TE Buffer 产品参数
产品名称：TE Buffer</p>
货号：lucigen MTE0970</p>
规格：70 ml</p>
存储温度：+4℃</p>
品牌：lucigen</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
lucigen MTE0970 TE Buffer 产品详情
Applications
Purification of TNA, DNA, or RNA for many applications, including PCR, RT-PCR, cloning, sequencing, Northern and Southern blotting, restriction analysis, and genomic library preparation.
The MasterPure™ Complete DNA and RNA Purification Kit uses a novel technology that enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material (Table 1 and Fig. 1).
The gentle salt-precipitation protocol permits rapid purification of high-molecular-weight nucleic acids from a single sample or many samples that are processed simultaneously (Fig. 4). High yields of nucleic acids are obtained using MasterPure Kits (Table 2), and yields and purity are highly consistent for different samples of the same material (Fig. 2). The resultant nucleic acids have an A260/A280 ratio of 1.8-2.0, indicating that they are substantially free of protein. The TNA, DNA, and/or RNA purified from different samples can be used directly in many applications. For example, the sensitivity of PCR or RT-PCR assays is often improved if the template is obtained using a MasterPure Purification Kit.
应用
用于许多应用的TNA，DNA或RNA的纯化，包括PCR，RT-PCR，克隆，测序，Northern和Southern印迹，限制性分析和基因组文库制备。
MasterPure™完整DNA和RNA纯化试剂盒采用了一种新技术，可以从各种生物材料中有效纯化完整的总核酸（TNA），DNA或RNA（表1和图1）。
温和的盐沉淀方案允许从单个样品或同时处理的许多样品中快速纯化高分子量核酸（图4）。使用MasterPure试剂盒获得高产量的核酸（表2），并且对于相同材料的不同样品，产率和纯度高度一致（图2）。得到的核酸的A 260 / A 280比率为1.8-2.0，表明它们基本上不含蛋白质。从不同样品中纯化的TNA，DNA和/或RNA可以直接用于许多应用中。例如，如果使用MasterPure纯化试剂盒获得模板，则PCR或RT-PCR测定的灵敏度通常会提高。
Benefits
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Purify TNA in 30 minutes without cumbersome columns.
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A260/A280 ratios consistently between 1.8 and 2.0.
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Recover >90% of theoretical DNA yield.
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No phenol, chloroform, or other caustic solvents.
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Each kit component is also available separately in a 10X package size.
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Low- and high-molecular-weight nucleic acids are recovered.
优点
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在30分钟内纯化TNA，无需繁琐的色谱柱。
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阿260 / A 280个比例一致1.8和2.0之间。
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恢复> 90％的理论DNA产量。
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不含酚，氯仿或其他腐蚀性溶剂。
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每个套件组件也可单独提供，封装尺寸为10X。
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回收低分子量和高分子量核酸。
lucigen MTE0970 TE Buffer 图示说明
Figure 1 (click to enlarge). Overview of the MasterPure™ Complete Kit protocol.
图1（点击放大）MasterPure™完整套件协议概述。
Figure 2. Comparison of PCR sensitivity using templates obtained using the MasterPure™ Complete DNA and RNA Purification Kit versus a spin-column kit. PCR amplification was performed after extraction from serial dilutions of E. coli cells ranging from 2 x 107 (lanes 1) to 200 (lanes 6). Lane M, DNA marker; lane 7, negative control.
图2.使用MasterPure™完整DNA和RNA纯化试剂盒与旋转柱试剂盒获得的模板比较PCR灵敏度。从大肠杆菌细胞的系列稀释液中提取后进行PCR扩增，范围为2×10 7（泳道1）至200（泳道6）。泳道M，DNA标记; 第7道，阴性对照。
Figure 3 (click to enlarge). DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure™ Complete Kit. M = kb ladder.
图3（点击放大）使用MasterPure™完整试剂盒从不同细胞来源纯化DNA，RNA和总核酸（TNA）。M = kb阶梯。
Figure 4. Consistent purification of DNA, TNA, and RNA. Five different genomic DNA (lanes 1-5), TNA (lanes 6-10), and total cellular RNA (lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure™ Complete DNA and RNA Purification Kit. Lane M, Kilobase
图4. DNA，TNA和RNA的一致纯化。用MasterPure TM Complete DNA和RNA纯化试剂盒从冷冻牛肝中分离5种不同的基因组DNA（泳道1-5），TNA（泳道6-10）和总细胞RNA（泳道11-15）样品。Lane M，Kilobase
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Human & mammalian RNA & genomic DNA
HIV
HCV
RSV
HPV
Yeast
Enterovirus
B. pertussis
E. coli
M. tuberculosis
Streptococcus mutans
Soy (tofu)
Maize
Insect tissues
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Plasma
Whole blood
Guthrie cards
Buccal cells
Liver
Mouse tail
Kidney
Saliva
Urine
Sputum
Tissue-culture cell lines
Cervical cells
Paraffin-embedded tissues
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Table 1. Examples of targets and samples analyzed using MasterPure™ DNA and RNA Purification Kits. Using an extremely simple salt-precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.
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HeLa/HL60 cells
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1 X 106 cells
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10-30
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3-12
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7-15
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Tissues
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Liver
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5 mg
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33-42
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5-10
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13-25
|
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Brain
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5 mg
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9-13
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6-9
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4-11
|
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Heart
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5 mg
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6-10
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4-7
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4-5
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Kidney
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5 mg
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10-17
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3-8
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14-17
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Thymus
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5 mg
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15-30
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6-12
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9-18
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Blood
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200 µl
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3-10
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3-9
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|
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Buffy coat
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300 µl
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40-55
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40-55
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3-6
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Mouse tail
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0.5 cm
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25-30
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9-11
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E. coli
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3.5 x 106 cells
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2.5-2.8
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1.3-1.6
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1.6-1.8
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S. mutans
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1.5 ml
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0.9
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|
|
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Yeast*
(S. cerevisiae)
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2.2 x 106 cells
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|
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11-18
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1.1 x 107 cells
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|
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70-78
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*For extraction of yeast DNA, the MasterPure™ Yeast DNA Purification Kit is recommended.
Table 2. Example of yields of nucleic acids obtained using MasterPure™ Complete DNA and RNA Purification Kits.
对此产品有兴趣，可联系中北林格获取更多详细信息。</p>
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