ScriptCap™ Cap 1 Capping System C-SCCS1710
产品简介：北京中北林格供应ScriptCap™ Cap 1 Capping System C-SCCS1710，中北林格是cellscript总代理，提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理，epicentre总代理，lucigen总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
ScriptCap™ Cap 1 Capping System C-SCCS1710 规格详情
名称：ScriptCap™ Cap 1 Capping System</p>
分类：Post-Transcriptional Capping</p>
货号：C-SCCS1710</p>
规格：10 reactions</p>
产品详情：For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods.</p>
供应商：中北林格</p>产地：us</p>发货地：北京</p>运输方式：送货上门/顺丰</p>是否现货：期货</p>
ScriptCap™ Cap 1 Capping System C-SCCS1710 产品详情
A. Synthesis of Cap 0 RNA from Uncapped RNA
1. The protocol below was designed to build a cap 1 structure on 5' end of 50-60 µg of uncapped RNA. If a cap 0 structure is desired, replace the ScriptCap 2'-O-Methyltransferase in Step 3 with an equivalent volume of RNase-Free Water. Combine the following reagents:
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Standard ScriptCap Cap 1 Capping Reaction (step 1)
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Component
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Amount
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RNase-Free Water
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x µl
|
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In vitro transcribed RNA, 50-60 µg
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≤67 µ
|
|
Total Volume
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67 µl
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2. Incubate at 65o C for 5-10 minutes, then transfer to ice.
3. While the heat-denatured RNA is cooling on ice, prepare a “Cocktail” of the following reaction components together in a separate tube.
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Standard ScriptCap Cap 1 Capping Reaction (step 3)
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|
Component
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Amount
|
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10X ScriptCap Capping Buffer
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10 µl
|
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10 mM GTP
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10 µl
|
|
20 mM SAM
|
2.5 µl
|
|
ScriptGuard RNase Inhibitor
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2.5 µl
|
|
ScriptCap 2'-O-Methyltransferase (100 U/µl)
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4 µl
|
|
Total Volume
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29 µl
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4. Just prior to starting the reaction, add the ScriptCap Capping Enzyme to the Cocktail from Step 3 and then combine this with the uncapped RNA solution from Step 1.
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Standard ScriptCap Cap 1 Capping Reaction (step 4)
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|
Component
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Amount
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Cocktailed reaction components (from step 3)
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29 µl
|
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ScriptCap Capping Enzyme (10 U/µl)
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4 µl
|
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Heat-denatured RNA (from step 1)
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67 µl
|
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Total Reaction Volume
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100 µl
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5. Incubate at 37o C for 30 minutes.
6. The Capped RNA can now be purified, or it can be 3' polyadenylated without purification by adding the reaction mixture directly to an A-Plus Poly(A) Polymerase Tailing Kit reaction (sold separately).
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ScriptCap™ Cap 1 Capping System Contents (10 Reactions)
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Kit Component
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Reagent Volume
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ScriptCap™ Capping Enzyme, 10 U/µl (red cap)
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40 µl
|
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ScriptCap™ 2'-O-Methyltransferase, 100 U/µl (blue cap)
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40 µl
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10X ScriptCap™ Capping Buffer. 0.5 M Tris-HCl, pH 8.0, 60 mM KCl and 12.5 mM MgCl2
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100 µl
|
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10 mM GTP Solution
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100 µl
|
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20 mM S-adenosyl-methionine (SAM)
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25 µl
|
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ScriptGuard™ RNase Inhibitor, 40 U/µl
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25 µl
|
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RNase-Free Water
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1.4 ml
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ScriptCap™ Cap 1 Capping System C-SCCS1710 关键词：
ScriptCap™ Cap 1 Capping System</p>
cellscript ScriptCap™ Cap 1 Capping System</p>
cellscript总代理 ScriptCap™ Cap 1 Capping System</p>
cellscript总代理中北林格 ScriptCap™ Cap 1 Capping System</p>
cellscript C-SCCS1710 10 reactions</p>
cellscript��代理 C-SCCS1710 10 reactions</p>
cellscript总代理中北林格 C-SCCS1710 10 reactions</p>
cellscript Post-Transcriptional Capping C-SCCS1710</p>
cellscript总代理 Post-Transcriptional Capping C-SCCS1710</p>
cellscript总代理中北林格 Post-Transcriptional Capping C-SCCS1710</p>
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