lucigen 60052-3 60061-2 Electrocompetent Cells
产品简介：北京中北林格供应lucigen 60052-3 60061-2 Electrocompetent Cells，中北林格是lucigen总代理，提供lucigen产品货号报价查询。其他品牌包括cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>
产品名称：E. cloni® 10G ELITE Electrocompetent Cells</p>
货号：lucigen 60052-3</p>lucigen 60061-2</p>
规格：24 rxns (SixPacks)</p>
存储温度：-80º C</p>
品牌：lucigen</p>
供应商：中北林格</p>
产地：us</p>
发货地：北京</p>
lucigen 60052-3 60061-2 Electrocompetent Cells 产品说明书
-
Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
-
Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
-
Available in a range of high transformation efficiencies (5 × 109 to 4 × 1010 cfu/µg).
-
Convenient packaging options.
标准克隆菌株的直接替代品（例如，DH5α™，DH10B™，JM109，TOP10，XL1-Blue等）
优化的遗传学，高产量：噬菌体T1抗性，核酸内切酶和重组减去，蓝/白筛选能力。
提供一系列高转换效率（5×109至4×1010 cfu /μg）。
方便的包装选择。
Looking for chemically competent version?
Free Sample Available
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability (Figures 1 and 2), while decreasing costs. These cells provide solutions for a wide range of applications at economical prices.
Lucigen 的 E. cloni 感受态细胞共享标准克隆菌株如DH5α™，DH10B™，JM109，TOP10等zui有用的遗传元件，并直接在克隆方案中取代它们。然而， E。cloni 电感受态细胞采用独特的制造技术，可提高转化效率，重组产量和可靠性（图1和图2），同时降低成本。这些电池以经济的价格为各种应用提供解决方案。
Choice of strain:
选择应变：
-
E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.
-
E. cloni 10GF′Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.
-
E. cloni 10G感受态细胞。 文库构建，克隆，亚克隆和质粒分离，有或没有蓝/白筛选。
-
E. cloni 10GF'Competent Cells。 含有用于感染M13的F'质粒以产生ssDNA。
Choice of efficiency:
-
E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
-
E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.
-
E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.
效率选择：
-
E. cloni 10G SUPREME电感受态细胞 > 4×1010 cfu / μgpUCDNA
SUPREME细胞具有zui高的转化效率，可从任何供应商处获得。选择SUPREME细胞用于zui苛刻的克隆情况，例如构建大型，高复杂性文库或克隆困难目标，这需要尽可能多的转化体。
-
E. cloni 10G和10GF'ELITE电感受态细胞> 2 × 10 10 cfu / μgpUCDNA ELITE细胞
与来自其他供应商的“超高效”细胞相比具有两倍的转化效率。ELITE细胞以较低的价格提供大量来自难以克隆的片段或有限DNA的转化体。
-
E. cloni 10G CLASSIC电感受态细胞 > 5 × 109 CFU /微克的pUC DNA
CLASSIC细胞是高效率电池，每反应一大大降低了成本。这些细胞是标准克隆和文库构建的zui经济的选择。10G CLASSIC电池有更大的封装尺寸，便于在更高容量的克隆应用中使用。
Custom Competent Cells
Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.
自定义���力细胞
Bulk Lucigen细胞或您的 大肠杆菌菌株，通过定制分配实现zui高的转染效率！有关 详细信息，请参阅 自定义能力单元页面。
Plasmid cloning. E. cloni 10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a leading supplier (Figures 1 and 2)
E. cloni 10G SUPREME细胞提供的重组产量高于领xian供应商提供的zui高效率细胞（图1和图2）
Figure 1. E. cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier. Both strains were transformed with 10 pg of pUC19 (n=16).
图1. E. cloni 10G SUPREME Electrocompetent Cells始终优于领xian供应商的“超高效”电池。用10μgpUC19（n = 16）转化两种菌株。
Figure 2.Transformation efficiency comparison of E. cloni 10G SUPREME Cells (Lucigen) and Mega DH10B cells (Invitrogen). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the Invitrogen cells)
图2.E.cloni 10G SUPREME细胞（Lucigen）和Mega DH10B细胞（Invitrogen）的转化效率比较 。根据制造商的说明用相同的pUC19 DNA对照（Invitrogen细胞提供）转化感受态细胞。
lucigen 60052-3 60061-2 Electrocompetent Cells 用于克隆和文库构建的E. cloni感受态细胞
Table 1. E. cloni Competent Cells for Cloning and Library Construction
E. cloni Cell Lines
|
Transformation Efficiency
(cfu/µg pUC DNA)
|
Cloning Methylated DNA
|
BAC, Cosmid Cloning
|
Blue/White Screening
|
|
10G SUPREME Electrocompetent
|
>4 × 1010
|
YES
|
YES
|
YES
without IPTG induction
|
|
10G ELITE Electrocompetent
|
>2 × 1010
|
YES
|
NO
|
YES
without IPTG induction
|
|
10G CLASSIC Electrocompetent
|
>5 × 109
|
YES
|
NO
|
YES
without IPTG induction
|
|
10GF´ ELITE Electrocompetent
|
>2 × 1010
|
YES
|
NO
|
YES
IPTG induction required
|
Efficiencies
-
E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA
-
E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA
-
E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA
对此产品有兴趣，可联系中北林格获取更多详细信息。</p>
- 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
- 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报