Anti-Ki67抗体(ab15580)

Anti-Ki67抗体(ab15580)
参阅全部 Ki67 一抗

描述

兔多克隆抗体to Ki67

宿主

Rabbit

特异性

Ab15580 is batch tested in ICC/IHC. A variability in IHC-Fr can occur with this antibody but we can guarantee stability in IHC-P. Some customers have successfully used ab15580 in Western Blot. Higher molecular weight proteins like ki67 may be more difficult to detect in WB. We recommend several potential optimisation steps: loading higher amounts of protein (20 ug and above), using lower percentage gels and/or Tris-Acetate gels, increasing antioxidant to maintain protein reduction, decreasing methanol and increasing SDS in the transfer buffer and increasing time and voltage of transfer. Larger proteins can be subject to degradation more than smaller proteins so lower molecular weight bands may be present. For further information or support please contact our Scientific Support Team.

经测试应用

适用于: IHC - Wholemount, IHC-P, IHC-FrFl, Flow Cyt, IHC-Fr, ICC/IF, ICC, IHC-FoFrmore details

种属反应性

与反应: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Human, Pig, Indian muntjac, Monkey, Chinese hamster, Common marmoset, Syrian hamster

**原

Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.

(Peptide available as ab15581.)

阳性对照