FOXO1A Rabbit Monoclonal Antibody
产品编号
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产品名称
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产品包装
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产品价格
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XY1600
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FOXO1A Rabbit
Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H, M, R, Z
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80.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
About this
Antibody
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Name
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FOXO1A Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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2308(Human);
56458(Mouse); 84482(Rat)
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SwissProt
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Q12778(Human);
Q9R1E0(Mouse); G3V7R4(Rat)
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Synonyms
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FKH1; FKHR;
FOXO1A
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Category
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PI3K/Akt/mTOR
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Background
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FKHR (for
forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family
of transcription factors. Transcriptional activation of FKHR proteins is
regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1
and results in FKHRL1 associating with 14-3-3 proteins and being retained in
the cytoplasm. Induction of apoptosis or withdrawal of growth factors
stimulates dephosphorylation and nuclear translocation of FKHR proteins,
leading to FKHR-induced gene-specific transcriptional activation. FKHR, also
designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed
protein that shuttles between the cytoplasm and nucleus. Genetic mutations in
FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly
found in alveolar rhabdomyosarcomas. These translocations result in the
fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and
homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which
contains a transcriptional activation domain. The Pax-3/FKHR fusion protein
appears to function as an oncogenic transcription factor that enhances the
activation of normal Pax-3 target genes.
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包装清单:
产品编号
|
产品名称
|
包装
|
XY1600
|
FOXO1A Rabbit
Monoclonal Antibody
|
50μl
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XY050
|
Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
FOXO1A Rabbit
Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。
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