Cleaved PARP Rabbit Monoclonal Antibody
产品编号
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产品名称
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产品包装
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产品价格
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XY1567
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Cleaved PARP Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF, ICC, FC
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H
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89.0kDa
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WB, Western
blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC,
Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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1:10-1:50
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1:50-1:200
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-
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
About this Antibody
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Name
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Cleaved PARP Rabbit Monoclonal Antibody
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Category
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Rabbit Monoclonal Antibody (RabMAb);
Primary antibody
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Isotype
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IgG
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Purification
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XYfinity purification
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About the Immunogen
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Immunogen
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Recombinant protein
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Gene ID
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142(Human)
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SwissProt
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P09874(Human)
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Synonyms
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ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP;
PARP-1; PPOL; pADPRT-1; PARP1
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Category
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Apoptosis
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Background
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Poly(ADP-ribose) polymerase-1 (PARP-1),
also designated PARP, is a nuclear DNA-binding zinc finger protein that
influences DNA repair, DNA replication, modulation of chromatin structure,
and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer
of ADP-ribose units from NAD(+) to a number of acceptor molecules including
chromatin. PARP-1 recognizes DNA strand interruptions and can complex with
RNA and negatively regulate transcription. Actinomycin D- and
etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a
p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor
(AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent
and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to
chromosomal instability due to higher frequencies of chromosome fusions and
aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the
efficient maintenance of genome integrity.This antibody recognizes the
apoptosis-specific 89 kDa catalytic domain fragment, but it does not
recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.
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包装清单:
产品编号
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产品名称
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包装
|
XY1567
|
Cleaved PARP Rabbit Monoclonal Antibody
|
50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Cleaved PARP
Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。
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