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- 产品名称:Recombinant Protein G, His-tag
- 产品型号:
- 产品展商:XYbscience
- 产品文档:无相关文档
简单介绍
Recombinant Protein G, His-tag由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附��的蛋白很难与管壁分离。Recombinant Protein G, His-tag而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
产品描述
Recombinant Protein G, His-tag
产品编号
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产品名称
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产品包装
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产品价格
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XY5030-1g
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Recombinant
Protein G, His-tag
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1g
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24768.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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-
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-
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-
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E. coli
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210aa
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29kDa
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His
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About this
Protein
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Name
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Recombinant
Protein G, His-tag (Recombinant Protein G, His-tag; Protein G, His-tag);重组蛋白G,His标签
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Synonyms
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N/A
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Purity
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>97% by
SDS-PAGE and RP-HPLC analyses
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Biological
Activity
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The
recombinant Protein G is a genetically engineered protein containing 3
IgG-binding regions of protein G. Cell wall binding region, cell membrane
binding region and albumin binding region have been removed from the
recombinant Protein G to ensure the maximum specific IgG binding. The
recombinant Protein G is ideal for purification of polyclonal or monoclonal
IgG antibodies. Protein G binds to various human, mouse and rat IgG
subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat
IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and
rabbit.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from additive free solution.
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Endotoxin
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Less than
0.1EU/μg of Protein G, His-tag as determined by LAL method.
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Reconstitution
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Dissolve in
distilled water or saline.
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Category
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Protein G
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Background
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Protein G is a
bacterial protein derived from the cell wall of certain strains of
b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of
various classes and subclasses of immunoglobulins from a variety of species.
Protein G binds to all IgG subclasses from human, mouse and rat species. It
also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
Protein G binds preferentially to the Fc portion of IgG, but can also bind to
the Fab region, making it useful for purification of F(ab') fragments of IgG.
Due to it's affinity for the Fc region of many mammalian immunoglobulins,
protein G is considered a universal reagent in biochemistry and immunology.
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Amino Acid
Sequence
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MGTYKLILNG KTLKGETTTE AVDAATAEKV
FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTE AVDAATAEKV
FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTK AVDAETAEKA
FKQYANDNGV DGVWTYDDAT KTFTVTEKLA AALEHHHHHH
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包装清单:
产品编号
|
产品名称
|
包装
|
XY5030-1mg
|
Recombinant
Protein G, His-tag
|
1mg
|
XY5030-10mg
|
Recombinant
Protein G, His-tag
|
10mg
|
XY5030-1g
|
Recombinant
Protein G, His-tag
|
1g
|
-
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说明书
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1份
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保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。
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