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组织蛋白酶S(CTSS)重组蛋白
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 性状冻干粉内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量25.5kDa
- 实际分子量-(差异分析请参阅说明书)
- 片段与标签Leu115~Ile331 (Accession # P25774) with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 纯度> 95%
- 等电点-
-
应用SDS-PAGE; WB; ELISA; IP
组织蛋白酶S(CTSS)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
组织蛋白酶S(CTSS)重组蛋白
Cathepsin Cathepsin Cathepsin Cathepsin S (CTSS)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
组织蛋白酶S(CTSS)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Leu115~Ile331 (Accession # P25774),
with N-terminal His-Tag.
Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Lysosome.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 7.1
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 25.5kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
组织蛋白酶S(CTSS)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-LPDSVD WREKGCVTEV KYQGSCGACW AFSAVGALEA QLKLKTGKLV
SLSAQNLVDC STEKYGNKGC NGGFMTTAFQ YIIDNKGIDS DASYPYKAMD QKCQYDSKYR
AATCSKYTEL PYGREDVLKE AVANKGPVSV GVDARHPSFF LYRSGVYYEP SCTQNVNHGV
LVVGYGD LNG KEYWLVKNSW GHNFGEEGYI RMARNKGNHC GIASFPSYPE I
