产品名称：Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体

Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体

• 来源单抗制备
• 宿主小鼠
• 效价(克隆号)A5
• Ig亚型IgG2b Kappa
• 纯化方式蛋白A + 蛋白G亲和纯化
• 标记物无标记物
• **原CPA573Hu21-Ⅲ型前胶原氨基端原肽(PIIINP)卵白蛋白偶联物
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度500ug/ml
• 且适物种-
• 应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM

  Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体特异性

  该抗体是针对PIIINP的小鼠单克隆抗体。在**组织化学染色和**印迹实验中能识别PIIINP。

  用法

  Western blotting: 0.5-5ug/ml

  Immunocytochemistry in formalin fixed cells: 5-30ug/ml

  Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml

  Immunohistochemistry in paraffin section: 5-30ug/ml

  Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

  Optimal working dilutions must be determined by end user.

  储存

  经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

  稳定性

  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

  Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体

  Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

  Organism Species: Homo sapiens (Human)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  11th Edition (Revised in May, 2016)

  [ PROPERTIES ]

  Source: Monoclonal antibody preparation

  Host: MouseⅢ型前胶原氨基端原肽(PIIINP)单克隆抗体

  Antibody isotype: IgG2b Kappa

  Purification: Protein A/G Affinity Chromatography. Clone number: A5

  Traits: Liquid

  Concentration: 500µg/mL

  UOM: 200µg

  Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]

  Immunogen: CPA573Hu21-OVA conjugated Procollagen III N-Terminal

  Propeptide (PIIINP)

  [ APPLICATIONS ]

  Western blotting: 0.5-5ug/ml

  Immunocytochemistry in formalin fixed cells: 5-30ug/ml

  Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml

  Immunohistochemistry in paraffin section: 5-30ug/ml

  Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

  Optimal working dilutions must be determined by end user. [ FORMULATION ]

  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. [ QUALITY CONTROL ]

  Content: The quality control contains recombinant PIIINP disposed in loading

  buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles. Store at 4

  oC for frequent use. Aliquot and store at -20Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体

  oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

  was determined by accelerated thermal degradation test, that is, incubate the

  protein at 37

  oC for 48h, and no obvious degradation and precipitation were

  observed.The loss rate is less than 5% within the expiration date under

  appropriate storage condition.[ IDENTIFICATION ]

  Figure 1. DAB staining on

  IHC-P

  Samples：

  A. Human Liver Tissue B. Human Kidney Tissue C. Human Glioma Tissue D. Human Stomach Tissue

  A B

  C D