谷氨酰胺酶(GLS)重组蛋白 GLS

谷氨酰胺酶(GLS)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 等电点-内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量38.1kDa
• 实际分子量-(差异分析请参阅说明书)
• 片段与标签Ile221~Leu533 with N-terminal His Tag
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 90%
• 应用SDS-PAGE; WB; ELISA; IP
  谷氨酰胺酶(GLS)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  谷氨酰胺酶(GLS)重组蛋白
  Recombinant Glutaminase (GLS)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  10th Edition (Revised in Jan, 2014)
  谷氨酰胺酶(GLS)重组蛋白[ PROPERTIES ]
  Residues: Ile221~Leu533
  Tags: Two N-terminal Tags, His-tag and T7-tag
  Accession: O94925
  Host: E. coli
  Subcellular Location: Cytoplasm, cytosol. Mitochondrion.
  Purity: >90%
  Endotoxin Level: <1.0EU per 1μg
  (determined by the LAL method).
  Formulation: Supplied as lyophilized form in PBS,
  pH7.4, containing 5% trehalose, 0.01% sarcosyl.
  Predicted isoelectric point: 5.9
  Predicted Molecular Mass: 38.1kDa
  Applications: SDS-PAGE; WB; ELISA; IP.
  (May be suitable for use in other assays to be determined by the end user.)
  谷氨酰胺酶(GLS)重组蛋白[ USAGE ]
  Reconstitute in sterile PBS, pH7.2-pH7.4.[ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8oC for one month.
  Aliquot and store at -80oC for 12 months.
  Stability Test: The thermal stability is described by the loss rate of the target
  protein. The loss rate was determined by accelerated thermal degradation test,
  that is, incubate the protein at 37oC for 48h, and no obvious degradation and
  precipitation were observed. (Referring from China Biological Products Standard,
  which was calculated by the Arrhenius equation.) The loss of this protein is less
  than 5% within the expiration date under appropriate storage condition.
  [ SEQUENCES ]
  The sequence of the target protein is listed below.
  IPDFMSFTSH IDELYESAKK QSGGKVADYI PQLAKFSPDL WGVSVCTVDG QRHSTGDTKV
  PFCLQSCVKP LKYAIAVNDL GTEYVHRYVG KEPSGLRFNK LFLNEDDKPH NPMVNAGAIV
  VTSLIKQGVN NAEKFDYVMQ FLNKMAGNEY VGFSNATFQS ERESGDRNFA IGYYLKEKKC
  FPEGTDMVGI LDFYFQLCSI EVTCESASVM AATLANGGFC PITGERVLSP EAVRNTLSLM
  HS CGMY DFSG QFAF HV G LPA KSGVAG GI L L VVP NVMGMMC WS PP L DKMG N
  SVKGIHFCHD LVSLCNFHNY DNL