RD-ES细胞 RD-ES

RD-ES细胞

数量： 大量

是否是肿瘤细胞： 1

物种来源： 人

ATCC Number： HTB-166™

相关**： 其他**

细胞形态： 上皮样

生长状态： 混合型生长

运输方式： 冻存运输

年限： 19 years

器官来源： 骨

Designations: RD-ES

RD-ES细胞Depositors: G Marshall, M Kirchen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed: adherent and clusters in suspension

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: bone

Disease: Ewing's sarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: Blood type B; Rh+

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1-2

RD-ES细胞PGM1, 1-2

PGM3, 1

Age: 19 years

Gender: male

Ethnicity: Caucasian

Comments: The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus.

Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter.

The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells.

Propagation: RD-ES细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:RD-ES细胞ATCC 30-2020