146-03E04 [146-3E4]细胞 146-03E04 [146-3E4]

产品价格: < 面议
产品型号:146-03E04 [146-3E4]
 牌:HZbscience
公司名称:上海沪震实业有限公司
  地:上海松江
发布时间:2018-07-18
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产品简介

146-03E04 [146-3E4]细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。146-03E04 [146-3E4]细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。

产品详细信息

146-03E04 [146-3E4]细胞

ATCC Number: CRL-2650™

运输方式: 冻存运输

数量: 大量

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 悬浮生长

细胞形态: **样

**类型: IgG1; kappa light chain

146-03E04 [146-3E4]细胞Designations: 146-03E04 [146-3E4]

Depositors: National Cancer Institute

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, but many cells attach

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:146-03E04 [146-3E4]细胞 immunoglobulin; monoclonal antibody; against a synthetic ras oncogene peptide

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a synthetic polypeptide with the sequence YTLVREIRQHKLRKLNPPDESGPGC corresponding to peptides 157 to 181 of the H-ras oncogene.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts with the synthetic H-ras oncogene peptide. It reacts with the Harvey (Ha-ras) p21 protein but not the Kirsten (Ki-ras) p21 protein.

Propagation: 146-03E04 [146-3E4]细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: 146-03E04 [146-3E4]细胞culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 21967: Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991

55710: Pumiglia K, et al. Raf-1 N-terminal sequences necessary for Ras-Raf interaction and signal transduction. Mol. Cell. Biol. 15: 398-406, 1995. PubMed: 7799948

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